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Prof. Dr. med. Eckhart Kämpgen
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Research Interests:
"Strategies of Cellular Immune Therapy"
Cellular Immunology
Selected References:
Schierer S, Hesse A, Knippertz I, Kaempgen E, Baur AS, Schuler G, Steinkasserer A, Nettelbeck DM. Human dendritic cells efficiently phagocytose adenoviral oncolysate but require additional stimulation to mature. Int J Cancer. 2012,130,7,1682-1694.
Gross S.; Vogel A.; Leisgang W.; et al. Exploring the feasibility of future combinatorial approaches of chemotherapy with immunotherapy for melanoma - influence of chemotherapeutic drugs on the functions of immune cells. EXPERIMENTAL DERMATOLOGY 2012,21,3,e36-e36
Hundorfean G.; Atreya R.; Agaimy A.; et al. Fluorescein-guided confocal laser endomicroscopy for the detection of ipilimumab-induced colitis.
ENDOSCOPY 2012,44,Suppl 2,E78-E79.
Gross S.; Hamann A.; Leisgang W.; et al. New lessons learned from cancer immunotherapy: tumor-specific CD4+Tcells frequently expand and react to short peptides. JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT 2011,9,9,770-771.
Vogel A.; Schoenhoefer D.; Schuler G.; et al. Detection and sorting of melanoma cells circulating in patients' blood. JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT 2011,9,9,794-794.
Gruesser K. V; Kaempgen E.; Sticherling M. Linear IgA Dermatosis in Association with Colitis ulcerative. JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT 2011,9,Suppl.1,219-219.
Gross S.; Schmid M.; Schierer S.; et al. Altered migration of E/L-Selectin expressing DC - a monitoring challenge. EXPERIMENTAL DERMATOLOGY 2011,20,2,188-189.
Schuler-Thurner B.; Erdmann M.; Voland S.; et al. Vaccination with class I and II tumor peptide-loaded, cocktail-matured monocyte-derived DCs demonstrates solid immunogenicity and prolonged survival in a subset of melanoma patients exhibiting a particular gene expression profile. EXPERIMENTAL DERMATOLOGY 2011,20,2,188-188.
Janetzki S, Price L, Britten CM, van der Burg SH, Caterini J, Currier JR, Ferrari G, Gouttefangeas C, Hayes P, Kaempgen E, Lennerz V, Nihlmark K, Souza V, Hoos A. Performance of serum-supplemented and serum-free media in IFNgamma Elispot Assays for human T cells. Cancer Immunol Immunother. 2010;59(4): 609-18
Erfurt-Berge C, Kaempgen E. [Melanoma and pregnancy]. Hautarzt. 2010;61(12): 1040-5.
Gross S.; Hamann A.; Schuler-Thurner B.; et al. Monitoring of vaccine-specific cytolytic, helper and regulatory T cells in melanoma patients after vaccination with autologous antigen-loaded dendritic cells. EXPERIMENTAL DERMATOLOGY 2010,19,2,189-189
Krug, C ; Birkholz, K ; Hombach, A ; Reuter, S ; Kershaw, M ; Kampgen, E ; Schwenkert, M ; Fey, G ; Schuler, G ; Abken, H ; Dorrie, J ; Schaft, N. Reprogramming T cells with cancer specificity by non-viral transfer of mRNA encoding recombinant immunoreceptors. HUMAN GENE THERAPY, 20 (11): 1531-1531 NOV 2009
Birkholz, K, Hofmann, C, Hoyer, S, Schulz, B, Harrer, T, Kampgen, E, Schuler, G, Dorrie, J, Schaft, N. A fast and robust method to clone and functionally validate T-cell receptors. JOURNAL OF IMMUNOLOGICAL METHODS, 346 (1-2): 45-54 JUL 31 2009
Knippertz, I, Hesse, A, Schunder, T, Kampgen, E, Brenner, MK, Schuler, G, Steinkasserer, A, Nettelbeck, DM. Generation of Human Dendritic Cells That Simultaneously Secrete IL-12 and Have Migratory Capacity by Adenoviral Gene Transfer of hCD40L in Combination With IFN-gamma. JOURNAL OF IMMUNOTHERAPY, 32 (5): 524-538 JUN 2009
Birkholz, K, Hombach, A, Krug, C, Reuter, S, Kershaw, M, Kampgen, E, Schuler, G, Abken, H, Schaft, N, Dorrie, J. Transfer of mRNA encoding recombinant immunoreceptors reprograms CD4(+) and CD8(+) T cells for use in the adoptive immunotherapy of cancer. GENE THERAPY, 16 (5): 596-604 MAY 2009
Voland, S ; Wiesinger, M ; van der Bruggen, P ; Schuler, G ; Kampgen, E. The galectin disaccharide ligand LacNAc prevents loss of tetramer staining after T cell activation. EXPERIMENTAL DERMATOLOGY, 18 (3): 298-298 MAR 2009
Gross, S ; Hamann, A ; Leisgang, W ; Hndle, I ; Schuler-Thurner, B ; Schuler, G ; Kampgen, E.
Getting T cells to work EUR+ transforming quantity into quality. EXPERIMENTAL DERMATOLOGY, 18 (3): 298-298 MAR 2009
Birkholz, K, Hombach, A, Krug, C, Reuter, S, Kershaw, M, Kampgen, E, Schuler, G, Abken, H, Dorrie, J, Schaft, N. Transfer of mRNA encoding recombinant immunoreceptors reprograms CD4+and CD8+T cells for the use in adoptive immunotherapy of cancer. EXPERIMENTAL DERMATOLOGY, 18 (3): 316-316 MAR 2009
Dorrie, J, Schaft, N, Wohn, CT, Wellner, V, Schunder, T, Muller, I, Kaempgen, E, Schuler, G. Lipofection or electroporation of RNA - consequences of different antigen-distribution on immunogenicity. EXPERIMENTAL DERMATOLOGY, 18 (3): 298-298 MAR 2009
Birkholz, K., Hoyer, S., Hofmann, C., Schulz, B., Kaempgen, E., Schuler, G., Schaft, N., and Dorrie, J. (2008). A fast and robust method to clone and test the function of T cell receptors. Experimental Dermatology 17, 258.
De Vleeschouwer, S., Ardon, H., Rutkowski, S., Kaempgen, E., Wolff, J., Wagner, S., Kramm, C., Van Calenbergh, F., and Van Gool, S. (2008). Dc-Based Immunotherapy for Relapsed High-Grade Glioma: Clinical Update of Cohorts A, B, C, and D from the Cohort Comparison Trial Hgg-Immuno-2003. Neuro-Oncology 10, 812.
De Vleeschouwer, S., Fieuws, S., Rutkowski, S., Van Calenbergh, F., Van Loon, J., Goffin, J., Sciot, R., Wilms, G., Demaerel, P., Warmuth-Metz, M., Soerensen, N., Wolff, J. E. A., Wagner, S., Kaempgen, E., and Van Gool, S. W. (2008). Postoperative adjuvant dendritic cell-based immunotherapy in patients with relapsed glioblastoma multiforme. Clinical Cancer Research 14, 3098-3104.
Dorrie, J., Schaft, N., Muller, I., Wellner, V., Schunder, T., Hanig, J., Oostingh, G. J., Schon, M. P., Robert, C., Kampgen, E., and Schuler, G. (2008). Introduction of functional chimeric E/L-selectin by RNA electroporation to target dendritic cells from blood to lymph nodes. Cancer Immunology Immunotherapy 57, 467-477.
Dorrie, J., Schaft, N., Wellner, V., Wohn, C., Schunder, T., Muller, I., Kampgen, E., and Schuler, G. (2008). Priming and boosting of melanA-specific T cells - in vitro studies on the importance of antigen-presentation density. Wiener Klinische Wochenschrift 120, 19.
Erdmann, M., Haendle, I., Schmitt-Haendle, M., Leisgang, W., Trump, S., Wiesinger, M., Theiner, G., Kaempgen, E., Schuler-Thurner, B., and Schuler, G. (2008). Comparison of immune responses after vaccination with tumour-peptide-loaded dendritic cells with or without prior denileukin diftitox treatment in metastasized malignant melanoma. Experimental Dermatology 17, 267.
Goldwich, A., Hahn, S. S. C., Schreiber, S., Meier, S., Kampgen, E., Wagner, R., Lutz, M. B., and Schubert, U. (2008). Targeting HIV-1 gag into the defective rihosomal product pathway enhances MHC class I antigen presentation and CD8(+) T cell activation. Journal of Immunology 180, 372-382.
Haendle, I., Schmitt-Haendle, M., Voland, S., Erdmann, M., Schliep, S., Schaft, N., Doerrie, J., Kaempgen, E., Schuler-Thurner, B., and Schuler, G. (2008). T-cell responses in stage IV melanoma patients vaccinated with dendritic cells electroporated with defined RNA. Experimental Dermatology 17, 263.
Heise, D., Schmid, M., Schaft, N., Doerrie, J., Schuler, G., and Kaempgen, E. (2008). Are TLR agonists better maturation stimuli for clinically applicable dendritic cell vaccines ? Experimental Dermatology 17, 264.
Knippertz, I., Hesse, A., Kaempgen, E., Schuler, G., Steinkasserer, A., and Nettelbeck, D. M. (2008). Induction of IL-12 secretion and migration of human dendritic cells by adenoviral gene transfer of CD40L and combined IFN-gammatreatment. Experimental Dermatology 17, 257.
Lehner, M., Schmid, M., Diessenbacher, P., Leverkus, M., Erfurt, C., Erdmann, M., Kaempgen, E., and Holter, W. (2008). Dendritic cell survival during differentiation and maturation depends on anti-apoptotic effects of TNF. Experimental Dermatology 17 , 263.
Mueller, I., Schaft, N., Doerrie, J., Grelle, I., Reiners, C., Becker, J. C., Schuler, G., and Kaempgen, E. (2008). Antigen specific abrogation of immunity using Indium111 labelled dendritic cells. Experimental Dermatology 17, 264.
Schaft, N., Krug, C., Reuter, S., Kosmides, D., Abken, H., Kershaw, M. H., Kaempgen, E., Schuler, G., and Dorrie, I. (2008). Making T cell independent of MHC - electroporation of RNA encoding chimeric T cell receptors. Experimental Dermatology 17, 258.
Schaft, N., Schunder, T., Krug, C., Reuter, S., Kosmides, D., Abken, H., Kershaw, M., Kampgen, E., Schuler, G., and Dorriee, J. (2008). Reprogramming T cells to become MHC independent - electroporation of RNA encoding chimeric antigen receptors. Wiener Klinische Wochenschrift 120, 8.
Schmitt-Haendle, M., Voland, S., Haendle, I., Kaempgen, E., Schuler-Thurner, B., and Schuler, G. (2008). Naturally occurring T-cell responses against the tumour antigens MageA3, MelanA and Survivin in stage IV melanoma patients. Experimental Dermatology 17, 251.
Schwenkert, M., Birkholz, K., Schwemmlein, M., Kellner, C., Kuegler, M., Peipp, M., Nettelbeck, D. M., Schuler-Thurner, B., Schaft, N., Dorrie, J., Ferrone, S., Kampgen, E., and Fey, G. H. (2008). A single chain immunotoxin, targeting the melanoma-associated chondroitin sulfate proteoglycan, is a potent inducer of apoptosis in cultured human melanoma cells. Melanoma Research 18, 73-84.
Erdmann M, Dörrie J, Schaft N, Strasser E, Hendelmeier M, Kämpgen E, Schuler G, Schuler-Thurner B
Effective clinical-scale production of dendritic cell vaccines by monocyte elutriation directly in medium, subsequent culture in bags and final antigen loading using peptides or RNA transfection.
J Immunother. 2007;30(6): 663-74
Wobser M, Voigt H, Houben R, Eggert AO, Freiwald M, Kaemmerer U, Kaempgen E, Schrama D, Becker JC
Dendritic cell based antitumor vaccination: impact of functional indoleamine 2,3-dioxygenase expression.
Cancer Immunol Immunother. 2007;56(7): 1017-24
Humrich JY, Thumann P, Greiner S, Humrich JH, Averbeck M, Schwank C, Kampgen E, Schuler G, Jenne L.
Vaccinia virus impairs directional migration and chemokine receptor switch of human dendritic cells.
Eur J Immunol. 2007 Apr;37(4):954-65.
Wobser M, Voigt H, Houben R, Eggert AO, Freiwald M, Kaemmerer U, Kaempgen E, Schrama D, Becker JC.
Dendritic cell based antitumor vaccination: impact of functional indoleamine 2,3-dioxygenase expression.
Cancer Immunol Immunother. 2006 Dec 29; [Epub ahead of print]
Dorrie J, Wellner V, Kampgen E, Schuler G, Schaft N.
An improved method for RNA isolation and removal of melanin contamination from melanoma tissue: Implications for tumor antigen detection and amplification.
J Immunol Methods. 2006 Jun 30;313(1-2):119-28. Epub 2006 May 16.
Humrich JY, Humrich JH, Averbeck M, Thumann P, Termeer C, Kampgen E, Schuler G, Jenne L.
Mature monocyte-derived dendritic cells respond more strongly to CCL19 than to CXCL12: consequences for directional migration.
Immunology. 2006 Feb;117(2):238-47.
Schadendorf D, Ugurel S, Schuler-Thurner B, Nestle FO, Enk A, Brocker EB, Grabbe S, Rittgen W, Edler L, Sucker A, Zimpfer-Rechner C, Berger T, Kamarashev J, Burg G, Jonuleit H, Tuttenberg A, Becker JC, Keikavoussi P, Kampgen E, Schuler G.
Dacarbazine (DTIC) versus vaccination with autologous peptide-pulsed dendritic cells (DC) in first-line treatment of patients with metastatic melanoma: a randomized phase III trial of the DC study group of the DeCOG.
Ann Oncol. 2006 Jan 17; [Epub ahead of print]
Schaft N, Dorrie J, Muller I, Beck V, Baumann S, Schunder T, Kampgen E,
Schuler G.
A new way to generate cytolytic tumor-specific T cells: electroporation of RNA coding for a T cell receptor into T lymphocytes.
Cancer Immunol Immunother. 2005 Dec 13;:1-10 [Epub ahead of print]
Berger TG, Strasser E, Smith R, Carste C, Schuler-Thurner B, Kaempgen E, Schuler G.
Efficient elutriation of monocytes within a closed system (Elutra) for clinical-scale generation of dendritic cells.
J Immunol Methods. 2005 Mar;298(1-2):61-72. Erratum in: J Immunol Methods. 2005 Aug;303(1-2):152.
Niels Schaft, Jan Dörrie, Peter Thumann, Verena E. Beck, Ina Müller, Erwin S. Schultz, Eckhart Kämpgen, Detlef Dieckmann, and Gerold Schuler
Generation of an Optimized Polyvalent Monocyte-Derived Dendritic Cell Vaccine by Transfecting Defined RNAs after Rather Than before Maturation
J Immunol 2005 174: 3087-3097.
Otto K, Andersen MH, Eggert A, Keikavoussi P, Pedersen LO, Rath JC, Bock M, Brocker EB, Straten PT, Kampgen E, Becker JC.
Lack of toxicity of therapy-induced T cell responses against the universal tumour antigen survivin.
Vaccine. 2005 Jan 4;23(7):884-9.
Eggert AO, Andersen MH, Voigt H, Schrama D, Kampgen E, Straten PT, Becker JC.
Characterization of mouse MAGE-derived H-2Kb-restricted CTL epitopes.
Eur J Immunol. 2004 Nov;34(11):3285-90.
Schrama D, Xiang R, Eggert AO, Andersen MH, Pedersen Ls LO, Kampgen E, Schumacher TN, Reisfeld RR, Becker JC.
Shift from systemic to site-specific memory by tumor-targeted IL-2.
J Immunol. 2004 May 15;172(10):5843-50.
Terheyden P, Schrama D, Pedersen LO, Andersen MH, Kampgen E, thor Straten P, Becker JC.
Longitudinal analysis of MART-1/HLA-A2-reactive T cells over the course of melanoma progression.
Scand J Immunol. 2003 Nov; 58(5): 566-71.
Leverkus M, McLellan AD, Heldmann M, Eggert AO, Brocker EB, Koch N, Kampgen E.
MHC class II-mediated apoptosis in dendritic cells: a role for membrane-associated and mitochondrial signaling pathways.
Int Immunol. 2003 Aug;15(8):993-1006.
Kolb-Maurer A, Kammerer U, Maurer M, Gentschev I, Brocker EB, Rieckmann P, Kampgen E.
Production of IL-12 and IL-18 in human dendritic cells upon infection by Listeria monocytogenes.
FEMS Immunol Med Microbiol. 2003 Apr 1;35(3):255-62.
Kammerer U, Eggert AO, Kapp M, McLellan AD, Geijtenbeek TB, Dietl J, van Kooyk Y, Kampgen E.
Unique appearance of proliferating antigen-presenting cells expressing DC-SIGN (CD209) in the decidua of early human pregnancy.
Am J Pathol. 2003 Mar;162(3):887-96.
Otto K, Kaempgen E, Dummer W, Broecker EB, Becker JC.
Late-type allergy to Ultravist (iopromid).
Allergy. 2003 Mar; 58(3): 261-2. No abstract available.
Schrama D, Pedersen LO, Keikavoussi P, Andersen MH, Straten Pt P, Brocker EB, Kampgen E, Becker JC.
Aggregation of antigen-specific T cells at the inoculation site of mature dendritic cells.
J Invest Dermatol. 2002 Dec;119(6):1443-8.
McLellan AD, Kapp M, Eggert A, Linden C, Bommhardt U, Brocker EB, Kammerer U, Kampgen E.
Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression.
Blood. 2002 Mar 15;99(6):2084-93.
Eggert AO, Becker JC, Ammon M, McLellan AD, Renner G, Merkel A, Brocker EB, Kampgen E.
Specific peptide-mediated immunity against established melanoma tumors with dendritic cells requires IL-2 and fetal calf serum-free cell culture.
Eur J Immunol. 2002 Jan;32(1):122-7.
Andersen MH, Keikavoussi P, Brocker EB, Schuler-Thurner B, Jonassen M, Sondergaard I, Straten PT, Becker JC, Kampgen E.
Induction of systemic CTL responses in melanoma patients by dendritic cell vaccination: cessation of CTL responses is associated with disease progression.
Int J Cancer. 2001 Dec 15;94(6):820-4.
